17200 - Total RNA Purification Kit

Features Purified high-quality RNA. Extract RNA from as little as a single cell. Isolate all RNA including siRNA and microRNA. No phenol extractions.

Quick Links Ordering Info Prod. Description Kit Specifications Key Figures Benefits Product Information Slideshow

Ordering Information

Item Cat. No. Kit Size Price (USD) Total RNA Purification Kit 17200 50 Please inquire

Product Description
Norgen’s Total RNA Purification Kit provides a rapid method for the extraction and purification of total RNA from cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi, plants and nasal or throat swabs. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA) without the use of phenol or chloroform.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Kit Specifications
Column Binding Capacity 50 ug Maximum Column Loading Volume 600 uL Size of RNA Purified All sizes Time to Complete 10 Purifications 20 minutes Average Yield:      HeLa Cells (1 x 10E6 cells) 15 ug    E. coli (1 x 10E9 cells) 50 ug    S. cerevisiae (1 x 10E7 cells) 30 ug	Maximum Amount of Starting Material: Animal Cells 3 x 10E6 cells Animal Tissues 25 mg Blood 100 uL Bacteria 1 x 10E9 cells Yeast 1 x 10E8 cells Fungi 50 mg Plant Tissues 50 mg

Key Figures

Figure 1. High Quality of Norgen's Isolated RNA Compared to a Competitor. Total E. coli RNA analyzed on the Agilent® 2100 BioAnalyzer. Note the presence of small RNA species (red circle) in the samples isolated via Norgen's kit and the absence of these RNA sp. in the comptitor RNA preparation.

Figure 2. Great Sensitivity. Total RNA was extracted from a decreasing number of 293 HEK cells followed by RT-PCR to detect isolated RNA. M is the marker lane.

Figure 3. Isolate a Diversity of RNA Species. Unlike most competitor’s kits (lanes C and D), Norgen's Total RNA Purification Kit (lane A and B) allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA. Lane M is Norgen's RNA Ladder. Total RNA, large and small RNA species, can be seen in lanes A and B (Norgen).

Figure 4. User-Friendly Procedure

Benefits

• All RNA species can be extracted, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
• Rapid spin-column format allows for the processing of 10 samples in 20 minutes.
• Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
• Total RNA has been isolated and detected from as little as a single cell.
• Extracted RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNAse protection and primer extension, and expression array analysis requiring the use of intact RNA.

US: 650-320-8669