21200 - Leukocyte RNA Purification Kit

Features High-quality RNA. High yields. Fractionate leukocytes in minutes. No phenol extractions.

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Ordering Information

Item Cat. No. Kit Size Price (USD) Leukocyte RNA Purification Kit 21200 50 $250.00

Product Description
Norgen’s Leukocyte RNA Purification Kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples. Selective isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. The purified RNA is of the highest quality and can be used in a number of downstream applications.

In this procedure the red blood cells are first removed from the sample through differential red blood cell lysis, and the leukocytes are recovered through centrifugation. The leukocytes are lysed, and purification of the leukocyte RNA is then based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. The RNA is preferentially purified from the other cellular components such as genomic DNA and proteins without the use of phenol or chloroform. The kit is able to isolate total leukocyte RNA, including all small RNA species.

Kit Specifications
Column Binding Capacity 50 ug Size of RNA Purified All sizes Minimum Blood Input 10 uL Maximum Blood Input 2mL or 3 x 10E6 leukocytes	Maximum Column Loading Volume 600 uL Average RNA Yield:      500 uL Human Blood 1.5 ug Time to Complete 10 Purifications 45 minutes

Key Figures

Figure 1. Leukocyte total RNA Leukocyte total RNA was isolated from 250 µL of rabbit blood from 4 different samples (lanes 1 to 4). Twenty µL of each elution was then run on a 1.5% formaldehyde-agarose gel. Lane M is Norgen's RNA Ladder.

Figure 2. Consistency and Yield vs. Competitor Leukocyte total RNA was isolated from 50 µL of human blood. Twenty uL out of 50 µL of each elution was then run on a 1.5% formaldehyde-agarose gel. Lane M is Norgen's RNA Ladder. Note the quality, consistency and yield of Norgen's kit (Lane 1 to 4) as compared to the competitor (Lane 5 and 6).

Figure 3. User-Friendly Procedure

Benefits

• Rapid spin-column format allows for the processing of 10 samples in 45 minutes.
• RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
• All leukocyte RNA species can be isolated, from large mRNA species down to microRNA (miRNA).
• Rapid removal of red blood cells from whole blood samples using differential red blood cell lysis.
• Purified RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNAse protection and primer extension, and expression array analysis requiring the use of intact RNA.

US: 650-320-8669