21300 - microRNA Purification Kit

Features Isolate all small RNAs. Rapid protocol. No phenol extractions. Remove large RNAs.

Quick Links Ordering Info Prod. Description Kit Specifications Key Figures Benefits Product Information Slideshow

Figure 1. HeLa microRNA Small RNA was isolated from HeLa cells using Norgen's microRNA Purification Kit (lanes C and D) and a competitor's kit (lanes A and B). Samples of the purified small RNA were run on an 8% urea-agarose gel. Lane M is Norgen's RNA ladder. Note that Norgen's kit is isolating only the small (< 200 nt) RNA species, with no contaminating larger RNA.

Figure 2. Efficient fractionation of the large from the small RNA species Norgen's microRNA Purification Kit was used to separate HeLa cell small RNA from the large RNA species. Essentially, total HeLa cell RNA is passed in the column under conditions that bind the large molecular weight RNA but not the small RNA. Samples were run on a formaldehyde-agarose gel to visualize the larger RNA species that are being removed. Lane M is a marker, Lanes A and B contain the large RNA and Lanes C and D contain the small RNA species.

Figure 3. User-Friendly Procedure

• Rapid spin-column format allows for the processing of 10 samples in 30 minutes.
• RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
• All small RNA species can be isolated including miRNA, siRNA, tRNA and 5S rRNA.
• Efficient isolation of small RNA species using a 2 column process, resulting in minimal contamination of larger RNA and genomic DNA.
• Purified RNA can be used in a number of downstream applications relating to gene regulation and functional analysis, including northern blotting and microarray analysis.