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22500 - Urine (Exfoliated Cell) RNA Purification Kit
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Features
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Ordering Information
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Product Description
Norgen’s Urine (Exfoliated Cell) RNA Purification Kit is designed for the rapid isolation and purification of total RNA from exfoliated cells that have been shed into the urine from the urinary tract. RNA from exfoliated cells can be used as non-invasive tools for a number of research applications including biomarker studies for the diagnosis and monitoring of bladder, kidney, or other urinary tract cancers. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The kit preferentially purifies RNA from other cellular proteinaceous components, as well as from the contaminating species found in urine such as glucose and salts.
* Yield varies due to the cell density of the sample
Key Figures
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| Figure 1. Isolation and Detection of RNA from the Exfoliated Cells in 50 mL of Urine RNA was isolated from the exfoliated cells in a 50 mL urine sample obtained from a healthy male using Norgen’s Urine (Exfoliated Cell) RNA Purification Kit. The isolated RNA was then subjected to reverse transcription and real-time PCR using primers to detect the ribosomal S14 gene. The red lines in the PCR baseline graph above correspond to RNA standards, while the blue lines correspond to the successful PCR results when RNA isolated from the exfoliated cells in 50 mL of urine were used as the template. |
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| Figure 2. User-Friendly Procedure |
Benefits
- Fast processing - Rapid spin-column format allows for the processing of multiple samples in 20 minutes.
- Isolate diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA.
- Isolate RNA from small samples - RNA can be isolated and detected from as little as 100 exfoliated cells.
- No phenol:chloroform extractions - Total exfoliated cell RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
- Recovered RNA is suitable for downstream applications - Purified RNA is fully compatible with real time PCR, reverse transcription PCR, Northern blotting, RNAse protection and primer extension, and expression array assays.
Applications
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