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23600 - RNA CleanUp and Concentration Kit
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Features
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Ordering Information
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Product Description
Norgen’s RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of up to 35 µg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Key Figures
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| Figure 1. Concentration and Detection of Increasing Amounts of HeLa RNA Increasing amounts of HeLa RNA in 50 µL input volumes were concentrated to 20 µL using Norgen’s RNA Clean-Up and Concentration Micro Kit. A 10 µL aliquot of the purified RNA was then used as the template in a qRT-PCR reaction to detect the S14 gene. The amounts indicated on the graph correspond to the amount of RNA that was used as the input for the qRT-PCR reaction, demonstrating the consistent performance of the kit even in the picogram range. |
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| Figure 2. High Quality RNA Total RNA was isolated from HeLa cells using TRI® Reagent, which relies on organic extractions. The resulting RNA was then purified using Norgen’s RNA Clean-Up and Concentration Micro Kit, and a portion of the purified RNA was then run on an Agilent 2100 Bioanalyzer to demonstrate the high quality of the purified RNA. |
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| Figure 3. User-Friendly Procedure |
Benefits
- Efficient clean-up from various enzymatic reactions - RNA clean-up from upstream enzymatic applications such as labeling, DNase treatment and in vitro transcription. Remove proteins, RNases, DNases, nucleotides, etc.
- Clean-up and concentration of RNA isolated by different methods - RNA that has been isolated using various methods, including phenol-chloroform extractions or alcohol precipitations, can be processed with this kit.
- Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes.
- No phenol:chloroform extractions - Norgen’s RNA Clean-Up and Concentration Micro Kit purifies RNA without the use of harmful chemicals such as phenol or chloroform.
- Isolate all small RNA molecules - All small RNA species can be isolated including microRNA, siRNA, tRNA, and 5S rRNA.
- Recovered RNA is suitable for downstream applications - Purified RNA can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array analysis.
Applications
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