24200 - RNA/microRNA/DNA/Protein Purification Kit

Features Purify RNA, microRNA, Protein and DNA from a single sample. No phenol extraction step. Fast and easy processing. Ideal for precious, difficult to obtain or small samples.

Quick Links Ordering Info Prod. Description Kit Specifications Key Figures Benefits Applications

Figure 1. Sequential Isolation of microRNA, Large RNA, Genomic DNA and Proteins from 1 x 10E6 HeLa Cells. Panels A and B show the separate isolation of large RNA and microRNA from 2 different samples of HeLa cells. Panel A is a 1X MOPS 1% agarose gel and Panel B is a 10% urea-PAGE gel. In both gels, Lane M is Norgen’s 1Kb RNA Ladder, Lanes 1 and 2 contain 3 uL out of the 50 uL elutions of the large RNA fraction, and Lanes 3 and 4 contain 3 uL out of the 50 uL elutions of the microRNA fractions. Panel C is a 1% agarose gel showing the gDNA isolated from the same 2 HeLa cell samples. Lane M is Norgen’s UltraRanger DNA Ladder and Lanes 1 and 2 contain 10 uL of each of the 100 uL elutions. Panel D is a 12% SDS-PAGE gel that contains the proteins that were isolated from the 2 HeLa cell samples. Lane M is a protein ladder and Lanes 1 and 2 contain 10 uL of the 100 uL elution of proteins that had been column purified. The large RNA, microRNA, gDNA and proteins are all intact and of the highest integrity and quality. Furthermore, the kit allows for the successful separate isolation of microRNA (<200 nt) and large RNA (>200nt).

Figure 2. Isolate High Quality RNA. RNA was isolated from HeLa cells using Norgen’s All-in-One Purification Kit and a leading market competitor for multiple analyte isolations. The purified RNA was used as the template in a qPCR reaction using GAPDH primers, and the results are shown in the PCR baseline graph to the left. The blue lines correspond to the PCR results when RNA isolated using Norgen’s kit was used as the template, while the green lines correspond to the results when RNA isolated using the competitors kit was used as the template. From the graph it can be seen that Norgen’s kit isolated RNA with a greater sensitivity, and that more RNA was isolated from the same input as evidenced by the lower CT values for the Norgen-isolated RNA.

Figure 3. User-Friendly Procedure

• Complete column purification - The RNA, DNA and proteins are all column purified without the use of phenol, chloroform or acetone.
• Isolate total or micro-enriched RNA - Two different protocols are provided to isolate either total RNA, including all sizes of RNA from large rRNA to microRNA, or separately isolate microRNA species (<200 nt) and large RNA species (>200 nt)
• Reduce variability - RNA, DNA and proteins are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability.
• Isolate from small samples - Sequential isolation of RNA, DNA and protein from a single sample. Ideal for precious, difficult to obtain or small samples.
• Rapid procedure - Isolate total RNA, genomic DNA and total proteins OR microRNA, large RNA, genomic DNA and proteins from a single sample in < 20 minutes.
• Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA, either separately or combined in a total RNA fraction.
• Process a wide range of sample types - Isolate total RNA, genomic DNA and proteins from cultured animal cells , tissue, blood, bacteria, yeast, fungi and plants.