24300 - Total RNA Purification 96-Well Kit

Features True total RNA isolation including miRNA and siRNA. No phenol extraction step. Highly sensitive. Fast and easy processing.

Quick Links Ordering Info Prod. Description Kit Specifications Key Figures Benefits Applications

Figure 1. Isolate Total RNA from a Diversity of Species Total RNA was isolated from 8 mg of brain (Lanes A), kidney (Lanes B), liver (Lanes C), lung (Lanes D) and spleen tissue (Lanes E), 7.5 x 10^5 HeLa (Lanes F) and CHO cells (Lanes G), and 5 x 10^8 bacteria (Lanes H) using Norgen’s Total RNA Purification 96-Well Kit. From observing the formaldehyde-agarose gel, it can been seen that Norgen’s kit can be used to successfully isolate total RNA, including small RNA species, from a broad range of sample types.

Figure 2. Consistent Isolation of Total RNA, Including microRNA Total RNA was isolated from samples of 5 x 10^5 HeLa cells using Norgen’s Total RNA Purification 96-Well Kit. Aliquots of each total RNA sample were then used in 2 different RT-qPCR reaction. In the first RT-qPCR the S15 gene of mRNA was amplified (Panel A), and in the second RT-qPCR reaction the miR-21 microRNA was amplified (Panel B). Both the mRNA and microRNA were amplified in a consistent manner from all the samples, with low variability of the C T values. Thus both types of RNA are being consistently isolated using this kit.

Figure 3. Sensitive RNA Isolation to <10 Cells RT-qPCR was used to detect mRNA isolated from various input amounts of HeLa cells using Norgen’s Total RNA Purification 96-Well Kit. The primers used for the RT-qPCR detected the S15 gene. Total RNA was isolated and detected linearly from as little as a single HeLa cell.

Figure 4. User-Friendly Procedure

• Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
• Isolate RNA from a wide range of samples - RNA can be isolated from cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi and plants (Figure 1).
• No phenol:chloroform extractions - Total RNA, including all small RNA species, is isolated without the use of harmful chemicals such as phenol or chloroform.
• Isolate RNA from very small samples - Total RNA has been isolated and detected from as little as a single animal cell (Figure 3).
• Fast and easy processing - 96-Well plates can be rapidly processed in 30 minutes using either a vacuum manifold or centrifugation format.
• Recovered RNA is suitable for many downstream applications - Purified RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNAse protection and primer extension, and expression array analysis requiring the use of intact RNA.