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24400 - Plant RNA/DNA Purification Kit
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Features
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Ordering Information
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Product Description
Norgen’s Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and genomic DNA simultaneously from a single sample of plants. The total RNA and genomic DNA are both column purified in under 30 minutes using a single column. It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. Traditionally the RNA and DNA would be isolated from different aliquots of a sample, however this novel technology will allow for their simultaneous isolation from the same sample. This will not only save time, but will also be of a great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.
Key Figures
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| Figure 1. Isolation of Total RNA and Genomic DNA from Tobacco, Tomato and Peach Leaf Tissue Total RNA and genomic DNA were isolated from 60 mg of tobacco leaf, 52 mg of tomato leaf and 56 mg of peach leaf using Norgen’s Plant RNA/DNA Purification Kit. Panel A is a 1X MOPS 1.5% agarose gel showing the total RNA that was isolated after the optional on-column DNase digestion. Lane M is Norgen’s 1 kb RNA Ladder, Lanes 1 and 2 contain RNA isolated from tobacco cells, Lanes 3 and 4 contain RNA isolated from tomato cells, and Lanes 5 and 6 contain RNA isolated from peach cells. Panel B is a 1.5% agarose gel containing the genomic DNA that was isolated after the optional on-column RNase digestion. Lane M is Norgen’s HighRanger 1kb DNA Ladder, Lanes 1 and 2 contain the tobacco DNA, Lanes 3 and 4 contain the tomato DNA and Lanes 5 and 6 contain the peach DNA. The RNA and DNA are intact and of the highest quality, and can be used in a number of different downstream applications. |
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| Figure 2. User-Friendly Procedure |
Benefits
- Complete column purification - The RNA and DNA are both column purified simultaneously using the same column.
- Reduce variability - RNA and DNA are isolated from a single plant sample with no splitting of the lysate, thus reducing inconsistent results and variability.
- Isolate from small samples - Simultaneous isolation of RNA and DNA from a single sample. Ideal for precious, difficult to obtain or small samples.
- Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA, without the use of phenol or chloroform.
- Isolate DNA-free plant RNA or RNA-free plant DNA - Optional protocols for on-column DNase or RNase digestion are provided if the user wishes to isolate pure, DNA-free RNA or pure, RNA-free DNA.
Applications
| RNA | Genomic DNA | |
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