25300 - FFPE RNA Purification Kit

Features Extract total RNA (including microRNA) from FFPE samples. No phenol extraction step. Includes DNAse. Isolated RNA is of high quality and integrity.

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Figure 1. High Quality and Yield of Total RNA Total RNA was isolated from equal amounts of an FFPE tissue sample using Norgen’s FFPE RNA Purification Kit and a leading competitors kit. The purified RNA was then resolved on an Agilent BioAnalzyer. As it can be seen, Norgen not only isolated higher yields of total RNA, but the RNA was also of a higher quality as evidenced by the higher RIN values obtained with Norgen’s RNA.

Figure 2. High Yield of a Diversity of RNA Species Total RNA was isolated from equal amounts of an FFPE tissue sample using Norgen’s FFPE RNA Purification Kit and a leading competitors kit. The purified RNA was then used as the template in a RT-qPCR for detecting the beta-actin gene (Panel A) and for detecting miR-21(Panel B). In both graphs the blue lines correspond to Norgen isolated-RNA and the green lines correspond to competitor-isolated RNA. As it can be seen, Norgen’s kit isolated higher yields of RNA in both cases, as indicated by the higher C T values of the blue lines. Also, Norgen’s kit successfully isolated not only large RNA (Panel A) but also microRNA (Panel B), indicating the diversity of RNA species isolated.

Figure 3. User-Friendly Procedure

• High quality and integrity of the isolated RNA - The purified total RNA is of the highest quality and integrity, and can be used in any sensitive downstream applications (Figure 1).
• Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
• High yields - Norgen’s FFPE RNA Purification Kit allows for the purification of high yields of total RNA (Figure 2).
• No phenol:chloroform extractions - Total RNA is isolated from FFPE tissue samples without the use of harmful chemicals such as phenol or chloroform.
• Rapid procedure - Isolate total RNA from FFPE tissue sections using a rapid spin column format.