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25800 - Plant/Fungi RNA Purification Kit
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Features
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Ordering Information
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Product Description
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including viral RNA, from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components, such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
* Yield varies due to the cell density of the sample
Key Figures
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| Figure 1. Isolation of High Quality RNA, even from Difficult Samples RNA was isolated from 50 mg samples of apple (Lanes 1), peach (Lanes 2), grape (Lanes 3), pine needle (Lanes 4), strawberry (Lanes 5) and pear (Lanes 6) using Norgen’s kit and a competitors kit. Norgen’s kit allowed for the isolation of high quality RNA from all the samples, including the difficult samples, while the competitor failed to isolate RNA from grape, pine needles and strawberry. Furthermore, only Norgen’s kit was able to isolate the small RNA species (white box). |
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| Figure 2. Detection of Plum Pox Virus in Peach Leaves Total RNA was isolated from two 50 mg samples of peach leaves using Norgen’s Plant/Fungi Total RNA Purification Kit, and 3 uL of the RNA was used in an RT-PCR reaction for the detection of plum pox virus. Plum pox virus was detected from both samples, indicating that the RNA is of a high quality and that the kit is highly sensitive for total RNA isolation. |
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| Figure 3. User-Friendly Procedure |
Benefits
- No liquid nitrogen required for homogenization - Liquid nitrogen is not required for homogenization of samples, making RNA purification rapid and convenient.
- Isolate RNA from a wide range of samples - Total RNA can be isolated from a wide range of plant and filamentous fungi samples.
- Isolate a diversity of RNA species - All sizes of RNA are isolated from large mRNA down to microRNA, without the use of phenol or chloroform.
- Isolate total RNA, including viral RNA - RNA samples can be used for the detection of viral pathogens, as viral RNA is isolated with the total RNA.
- High yield of RNA - High yields of purified RNA can be isolated with this kit.
- No phenol:chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform. RNA is of the highest quality and can be used in a number of downstream applications.
Applications
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