26400 - Water RNA/DNA Purification Kit

Features Isolate total DNA and RNA from all microorganisms found in water, including bacteria, fungi and algae. RNA and DNA are both column purified simultaneously using the same column. Elution contains concentrated DNA and RNA without the need for further precipitation. Complete RNA (including microRNA) without phenol. Isolated RNA and DNA is of high quality and integrity for all downstream applications.

Quick Links Ordering Info Prod. Description Kit Specifications Key Figures Benefits Applications

Ordering Information

Item Cat. No. Kit Size Price (USD) Water RNA/DNA Purification Kit - 0.22 um 26400 25 Please inquire Water RNA/DNA Purification Kit - 0.45 um 26450 25 Please inquire

Product Description
Norgen’s Water RNA/DNA Purification Kit provides a convenient and rapid method for the detection of microorganisms from environmental water samples. The kit allows for the rapid isolation and purification of total RNA and DNA simultaneously from the microorganisms found in different types of water samples. The total RNA and DNA (including genomic DNA) are isolated from all the microorganisms found in the water, including bacteria, fungi and algae without the use of any inhibitory organic substances. The microorganisms are captured by passing the water sample through either a 0.22 µm or a 0.45 µm filter, and are subsequently lysed using the provided Bead Tubes. Both the RNA and DNA are then column purified in under 45 minutes using a single column. The purified RNA and DNA are highly concentrated, and can be used directly in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, Southern blotting and sequencing reactions.

Kit Specifications
Minimum Water Input 10 mL Maximum Water Input 100 mL Elution Volume 100 uL	Maximum Filter Column Loading Volume 600 uL Maximum Spin Column Loading Volume 100 uL Time to Complete 10 Purifications 45 minutes

Key Figures

Figure 1. High Yield and Purity of RNA and DNA Total RNA and DNA were isolated from a water sample using Norgen’s Water RNA/DNA Purification Kit and subsequently run on gels for visual analysis. In Panel A 10 uL aliquots of the elution were run on a 1% TAE agarose gel, and in Panel B 5 uL aliquots of the elution were run on a 1.5% formaldehyde agarose gel. From observing the gels it can be seen that the kit allows for the isolation and purification of high yields of concentrated and high quality RNA and DNA.

Figure 2. Detection of E. coli 16S rDNA by Real-Time PCR E. coli was spiked into 50 mL water samples at increasing concentrations and the RNA and DNA were isolated using Norgen’s Water RNA/DNA Purification Kit. The RNA/DNA elution was then used as the template in a real-time PCR reaction using E. coli 16S rDNA primers. The E. coli was successfully detected in all cases, indicating the high sensitivity of the kit for detecting microorganisms within the water.

Figure 3. User-Friendly Procedure

Benefits

• Rapid detection of microorganisms in water samples - Isolate total DNA and RNA from all microorganisms found in the water, including bacteria, fungi and algae.
• Complete column purification - The RNA and DNA are both column purified simultaneously using the same column.
• Fast and easy processing - Rapid spin column format allows for the isolation of both RNA and DNA in under 45 minutes.
• Isolate highly concentrated RNA and DNA - The purified RNA and DNA is eluted in low volumes, allowing for the direct use of the nucleic acid in downstream applications
• Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA, without the use of phenol or chloroform

Applications

quantitative, real-time PCR quantitative, real-time RT-PCR conventional PCR reverse transcription PCR Northern blotting Southern blotting Sequencing

US: 650-320-8669