Human OneArray™ Microarray
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Features
- 30,968 human genome probes and 1082 experimental control probes
- Highly sensitive 60-mer sense-strand polynucleotide probes
- 1" X 3" (25mm x 75mm) standard-format glass slide
- Latest information of human genome content from public domains is used to design the probes that monitor the expression level of corresponding human protein-coding genes
- Each probe is spotted onto the array in a highly consistent manner using an innovative non-contact spotting technology adapted and perfected by Phalanx Biotech for microarray manufacturing.
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| Ordering Information |
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| Item |
Cat. No. |
# of Arrays |
Acad. Price |
Comm. Price |
| Human Whole Genome OneArray™ Microarray |
HOA_004 |
10 |
$ 990 USD |
$ 1490 USD |
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*For 100 array quantities, please inquire about our bulk discounts.
| OneArray™ Advantages |
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- 100% satisfaction & replacement guarantee
- Outstanding customer and technical support team
- Competes head-to-head with popular platforms
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- Highly competitive pricing << More>>
- Specific 60-mer oligonucleotide probes
- Uniform, non-contact printing technology
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| A |
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| B |
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| Figure 1: Post Hybridization of Human OneArray using dual colors, indirect labeling of aRNA. A) shows the full microarray; B) is a magnified region to show clarity and spot morphology. |
| Probe Set Content |
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- Each 60-mer oligonucleotide probe is designed to hybridize to a specific target gene described in the latest public domain contents, such as Unigene, Cancer Genome Anatomy Project (CGAP), BioCarta, Kyoto Encyclopedia of Gene and Genome (KEGG), and validated by the Human Genome Sequencing Project.
- The most well-known and studied target genes are the first priority of probe selection.
- 1082 performance monitoring control probes for hybridization, sample quality, and labeling reactions.
- Also included are the ArrayControl™ Sense Oligo Spots by Ambion.
- Downloadable gene lists, probe sequences, and whole genome GAL files. << More>>
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| Human OneArray™ Probe Set Content |
| Probe Type |
Number of Probes |
| UniGene and Refseq based |
30,968 (total)* |
UniGene build #175-based and/or Refseq based with Entrez Gene ID including:
CGAP (Cancer Genome Anatomy Project)
Biocarta and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways |
24,858 |
| Unigene Build #163-based with Gene ID and experimentally selected |
2265 |
*Human OneArray is guaranteed to have > 98% of the total probe content printed.
Table 1 (above): Human Genome Content |
| Human OneArray™ Performance |
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Table 2 (right) displays results obtained using 10 Human OneArray DNA Microarrays with 5 ug of total RNA from the Universal Reference RNA from Stratagene with one round of amplification using Amino Allyl MessageAmp™ Kit from Ambion.
For Hybridization, 10 ug of aRNA was labeled with Cyanine 5 fluorescent, and scanned with an Agilent scanner*. |
| Human OneArray™ Whole Genome Hybridization |
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Spot Number |
Spot Percentage |
Average Signal Intensity |
| Average Value |
27,425 |
88.6 |
1527.5 |
| CV |
1.5% |
1.5% |
7.4% |
Table 2 (right): Human OneArray Repeatability
To learn more about the OneArray advantages, visit The Power of OneArray. << More>> |
*Agilent scanner data:
PMT: 100/10;
Filter: F635.Median - B636.Mean >2.6 B635 sd. |
| Control Features |
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