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Spot |
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Welcome to Spot, Phalanx Biotech's monthly guide to getting the most out of your microarray experiments. |
November 2007
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What’s new with OneArray™: Thank you for becoming a OneArray user Holiday Deals – let us do microarray experiments for you over the holidays >>Learn More Bulk pricing for core labs and consortiums Get a free OneArray pointer for your next presentation—just write to Spot and give your feedback Ask Spot: How does OneArray’s 100% satisfaction guarantee work? What do I do if I do not have a sufficient amount of RNA for OneArray service? How do I optimize my pre-hybridization? Where can I get the .gal files for an Agilent scanner? |
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Thank you for becoming a OneArray user. Since OneArray’s launch in October, we have received tremendous interest from the microarray community! Visit our Web site to see the growing list of satisfied OneArray users. Your response encourages us to continue our efforts to enable you to do all the experiments you want to do–more time points, more samples, more repeats–with no additional strain on your budget or compromise in the quality of your data. And all of our products and services are backed by a 100% satisfaction guarantee.
Now getting OneArray can be as simple as walking to the lab next door. If you have a core facility, encourage them to become a OneArray Partner. If you do not have a microarray core facility, you and your colleagues can also become a OneArray Partner. Not only will you have a convenient supply of OneArray microarrays, but partnership will enable you to buy in bulk quantities and get the best prices. We will work with you to find a solution that brings OneArray to your laboratory at the lowest cost. Contact us for more information.
How does OneArray’s 100% satisfaction guarantee work?
Spot says: All of our products and services are backed by a 100% satisfaction guarantee. Simply contact us at spot@phalanxbiotech.com or (650)320-8669 and we will provide you with a replacement OneArray or a refund. If you are unhappy with the service we have provided for you, contact us and pay nothing. (For more details please read the product user manual and service contract).
What steps can I take if I do not have a sufficient amount of total RNA? (For service users)
Spot says: I am willing to work with you: contact me, I may have a solution.
How do I optimize my pre-hybridization? (For end users)
Spot says: Slide preparation is essential for eliminating high background signal. Here are a few tips for effective pre-hybridization of OneArray microarrays:
1. Recent changes in the pre-hybridization protocol can improve spot homogeneity and reduce high background signal. Our most recent modification incorporates an ethanol pre-wash that greatly improves the quality of data. Always check for the latest protocol on our Web site Support page.
2. Excessive jarring or mishandling of the microarrays can lead to aberrant spot morphology. This is often times the result of impact or extreme shear forces at the surface of the microarray. The ethanol pre-wash (see above) acts to eliminate some of these susceptibilities; careful handling is always recommended.
3. Unlike many other protocols, our pre-hybridization solution does not contain formamide. PhalanxBio scientists have found formamide can contribute to high background signal if included in the pre-hybridization mixture. (Howerver, keep in mind that fresh, de-ionized formamide IS included in the hybridization solution.)
4. A good source of BSA is essential for efficient slide preparation. Use at least molecular biology grade BSA (for example, electrophoresis grade is not effective). In addition, check to ensure you are using 1% BSA for pre-hybridization; this corresponds to 10mg/mL.
For more troubleshooting, information, and answers to your questions, please visit our support page. If you have specific questions, do not hesitate to contact us directly at 650-320-8669 ext. 111 or spot@phalanxbiotech.com.
Where can I get the array files for an Agilent® scanner? (For end users)
Spot says: I have prepared .gal files compatible with Agilent® scanners in addition to the existing files compatible with GenePix™ software. Email spot@phalanxbiotech.com.
àJust send us your RNA, and take your vacation–we will work while you relax. We are offering a 20% discount on services to first-time customers for orders placed by the end of 2007. Now we are offering the same discount to you, too. All service pricing includes the cost of the microarrays.
àPlanning your experiments for the next year? Pricing on any service order placed before the end of 2007 will be honored through the end of 2008. To order, contact our sales department at 650-320-8669.
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Human OneArray |
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Single Dye |
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Number of Samples |
Number of Arrays |
Product ID Number |
Regular Price |
First-time Customer Price |
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1 |
2 (duplicate) |
HSP_0012 |
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$280 |
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1 |
3 (triplicate) |
HSP_0013 |
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$360 |
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Dual Dye |
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2 |
2 (duplicate) |
HDP_0022 |
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$400 |
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Mouse OneArray |
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Single Dye |
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Number of Samples |
Product ID Number |
First-time Customer Price |
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1 |
2 (duplicate) |
MSP_0012 |
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$280 |
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1 |
3 (triplicate) |
MSP_0013 |
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$360 |
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Dual Dye |
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2 |
2 (duplicate) |
MDP_0022 |
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$400 |
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Feedback |
If you have any questions you would like answered, or would like tips on other aspects of gene expression profiling using OneArray, please Email us at spot@phalanxbiotech.com.
If you would like to be removed from this mailing list, please reply to this Email with “Unsubscribe” (no quotes) in the subject line.
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Phalanx Biotech Group, Inc. |
Ph: 650-320-8669 |
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1400 Page Mill Road |
Fax: 650-320-8488 |
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Building B |
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Palo Alto, CA 94304 |
OneArray™, Human OneArray™, and Mouse OneArray™ are trademarks of Phalanx Biotech Group, Inc. in the United States and other countries. All trademarks and copyrights used in this publication belong to their respective owners and are the sole property of their respective owners.
©2007 Phalanx Biotech Group, Inc.