RNA/DNA/Protein Purification Kit - 23500

Features

Sequentially isolate and purify total RNA, genomic DNA and total proteins from a single sample.
Ideal for small or difficult to obtain samples.
Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants.
Rapid and efficient spin column procedure.

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Ordering Info
Prod. Description
Kit Specifications
Key Figures
Benefits
Applications

Product Information Slideshow

Ordering Information

Item	Cat. No.	Kit Size	Price (USD)
RNA/DNA/Protein Purification Kits	23500	20	$205.00

Product Description
Norgen's RNA/DNA/Protein Purification Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. The total RNA, genomic DNA and proteins are all column purified in less than 20 minutes using Norgen's proprietary resin as a separation matrix. This kit is ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen's RNA/DNA/Protein Purification Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purification procedure is very rapid, allowing for the isolation of total RNA, genomic DNA and proteins from a single sample in less than 30 minutes. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

Kit Specifications

Column Binding Capacity (RNA)	50 ug
Column Binding Capacity (DNA)	20 ug
Column Binding Capacity (Protein)	200 ug
Size of RNA Purified	All sizes
Size of DNA Purified	> 30 kbp

Average Yield:
HeLa Cells (1 x 10E6 cells)	15 ug RNA
HeLa Cells (1 x 10E6 cells)	8 ug DNA
HeLa Cells (1 x 10E6 cells)	150 ug protein
Time to Complete 10 Purifications	30 minutes

Key Figures

Figure 1. Sequential Isolation of Total RNA, Genomic DNA and Proteins from 5 x 10^5 of HeLa Cells. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of HeLa cells. Lane M is Norgen's 1Kb RNA Ladder, and Lanes 1 and 2 contain 3 µL out of the 50 µL elutions. Panel B is a 1% agarose gel showing the gDNA isolated from the same 2 HeLa cell samples. Lane M is Norgen's UltraRanger 1 kb DNA Ladder and Lanes 1 and 2 contain 10 µL of each of the 100 µL elutions. Panel C is a 12% SDS-PAGE gel that contains the proteins that were isolated from the 2 HeLa cell samples. Lane M is a protein ladder and Lanes 1 and 2 contain 10 µL of the 100 µL elution of proteins that had been column purified. The RNA, gDNA and proteins are all intact and of the highest integrity and quality.

Figure 2. High Quality RNA and DNA RNA isolated from HeLa cells was used as the template in an RT reaction with and without reverse transcriptase in the absence of DNase treatment. The RT reaction was then used in an RT-PCR to detect the GAPDH gene. There was no amplification in the absence of reverse transcriptase (Lanes 2 and 4), indicating that the RNA is free of genomic DNA. Lanes 1 and 3 contain the successful RT-PCR results when reverse transcriptase was used. Genomic DNA was also successfully used in a PCR reaction using the same set of primers to detect the GAPDH gene (Lanes 5 and 6), indicating the high quality of the isolated DNA. Larger PCR product is obtained due to extra intron in the genomic DNA. Lane M is Norgen's CloneSizer 100bp DNA Ladder and Lane 7 is a control with no template. Therefore RNA and DNA isolated using the kit are high quality and can be used in downstream applications.

Figure 3. High Quality Total Proteins Total proteins were isolated from HeLa cells and column purified. Western blot analysis of duplicate samples with antibodies against GAPDH demonstrate the proteins are of a high quality and can be used in common downstream applications. Lane 3 is a negative control.

Figure 4. User-Friendly Procedure

Benefits

Complete column purification - The RNA, DNA and proteins are all column purified using the same column.
Reduce variablility - RNA, DNA and proteins are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variablility.
Isolate from small samples - Sequential isolation of RNA, DNA and protein from a single sample. Ideal for precious, difficult to obtain or small samples such as biopsy material or single foci from cell cultures.
Rapid procedure - Isolate total RNA, genomic DNA and total proteins from a single sample in less than 20 minutes.
Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA.
Isolate high quality RNA, DNA and proteins - The purified RNA, DNA and proteins are of the highest quality and can be used in a number of downstream applications.

Applications

RNA	Genomic DNA	Protein
Real time PCR Reverse transcription PCR Northern blotting RNAse protection Primer extension Expression array analysis	PCR Sequencing Southern blotting SNP analysis	SDS-PAGE analysis Western blots Mass Spectrometry