FFPE RNA/DNA Purification Kit - 25000

Features
  • Extract total RNA (including microRNA) and genomic DNA separately or together.
  • No phenol extraction step.
  • Purified nucleic acids are well suited for qRT-PCR, qPCR, mutation screening, and microarray analysis.
  • Isolated RNA and DNA are of high quality and integrity.
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Ordering Information
Item Cat. No. Kit Size Price (USD)
FFPE RNA/DNA Purification Kit 25000 50 $263.00

Product Description
Norgen's FFPE RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Alternatively, the kit can be used to isolate total RNA alone or genomic DNA alone from FFPE tissue samples through varying the protease digestion time and performing optional RNase or DNAse digestions . Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen's FFPE RNA/DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids.

Kit Specifications
Column Binding Capacity (RNA) 50 ug
Column Binding Capacity (gDNA) 15 ug
Size of RNA Purified All sizes
Maximum Amount of Starting Material:
Paraffin Slices 5 slices 20 um thick
Unsectioned Block 25 mg

Key Figures
Figure 1. High Quality and Yield of Total RNA Total RNA was isolated from equal amounts of an FFPE kidney sample using Norgen's FFPE RNA/DNA Purification Kit and a leading competitors kit. The purified RNA was then resolved on an Agilent BioAnalzyer. As it can be seen, Norgen not only isolated higher yields of total RNA, but the RNA was also of a higher quality as evidenced by the higher RIN values obtained with Norgen's RNA.
Figure 2. High Quality Genomic DNA Genomic DNA was isolated from an FFPE kidney tissue sample using Nogen's FFPE Nucleic Acid Purification Kit in duplicate and run on a 1.5% agarose gel. From observing the gel it can be seen that the kit isolates high yields of intact genomic DNA. Lane M is Norgen's HighRanger 1kb DNA Ladder, and Lanes 1 and 2 contain the purified DNA samples.
Figure 3. User-Friendly Procedure

Benefits
  • High quality and integrity of the isolated nucleic acid - Both the purified total RNA and/or genomic DNA are of the highest quality and integrity, and can be used in any sensitive downstream application (Figures 1 and 2).
  • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
  • High yields - Norgen's FFPE RNA/DNA Purification Kit allows for the purification of high yields of total RNA or DNA (Figure 1).
  • Rapid procedure - Isolate total RNA and/or genomic DNA from FFPE tissue sections using a rapid spin column format.
  • Versatile procedure - The kit allows for the purification of either pure total RNA , pure genomic DNA or both RNA and genomic DNA through the use of tailored protease digestion times.

Applications
  • qPCR
  • qRT-PCR
  • primer extension
  • expression array assays
  • PCR reactions
  • sequencing
  • Southern blotting
  • SNP analysis