Total RNA Purification Maxi Kit - 26800

Features
  • Isolate total RNA from a wide variety of sources.
  • Purify high-quality RNA in 40 minutes.
  • Isolate all RNA including siRNA and microRNA.
  • No phenol extractions.
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Ordering Information
Item Cat. No. Kit Size Price (USD)
Total RNA Purification Maxi Kit 26800 8 $132.00

Product Description
Norgen's Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), without the use of phenol or chloroform. Norgen's kit is the only kit on the market that isolates true total RNA, as other kits must use phenol to recover all sizes of RNA. Therefore Norgen's kit offers significant advantages in functionality, savings on cost, ease-of-use, no hazerdous organic waste, and no inhibitory effect on PCR amplifications as a result of residual phenol. With this kit both microRNA and mRNA can be studied from the same sample without further purifications, thus offering considerable advantages when comparing and relating experssion of microRNA to other RNA.

Purification is based on spin column chromatography using Norgen's proprietary resin as the separation matrix. Briefly, the cells or tissue of interest are first lysed using the provided Lysis Solution, ethanol is added and the RNA is bound to Norgen's maxi column (BIND). Under these conditions only the RNA will bind to Norgen's resin while most of the contaminating cellular proteins are removed in the flowthrough or retained on top of the resin. The bound RNA is then washed to remove any remaining impurities (WASH). Lastly, the purified total RNA is eluted into 2 mL of the provided Elution Buffer or water (ELUTE).

Norgen's proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (microRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, microRNA cloning and amplification and Next Generation Sequencing.

Kit Specifications
Column Binding Capacity 1.5 mg
Maximum Column Loading Volume 20 mL
Size of RNA Purified All sizes
Time to Complete 4 Purifications 40 minutes
Average Yield:  
   HeLa Cells (5 x 10E7 cells) 750 mg
   E. coli (2.5 x 10E10 cells) 1.5 mg
    
Maximum Amount of Starting Material:
Animal Cells 5 x 10E7 cells
Animal Tissues 50 - 250 mg
Blood 2 - 10 mL
Bacteria 2.5 x 10E10 cells
Yeast 2 x 10E8 cells
Fungi 1 g
Plant Tissues 1 g

Key Figures
Figure 1. Isolate a Diversity of RNA Species. Total RNA was isolated from 10 mL of an overnight E. coli culture using Norgen's Total RNA Purification Maxi Kit. One microliter of the 2 mL elution was analyzed on an Agilent® 2100 BioAnalyzer RNA Nano 6000 chip. As it can be seen, high quality total RNA was isolated with minimal degradation. As well, a large amount of small RNA (<200 nt) was recovered, indicating that the kit is able to isolate a diversity of RNA species.
Figure 2. Good Amplification and Consistency of Isolation. Norgen's Total RNA Purification Maxi Kit was used to isolate total RNA from six different overnight samples of E. coli (10 mL samples). In brief, one microgram of the isolated RNA was subjected to a 20 µL reverse transcription using Invitrogen Superscript III. Three microliters of the reverse transcription was used as the template in a 20 µL qPCR reaction using 16S rRNA E. coli specific primers and Bio-Rad iQ mix. As it can be seen, the RNA was detected and successfully amplified in all cases. Furthermore, all samples showed a similar CT value, indicating consistent isolation..
Figure 3. User-Friendly Procedure

Benefits
  • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
  • Fast and easy processing - Rapid spin-column format allows for the processing of 4 samples in under 40 minutes.
  • No phenol:chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
  • Isolate total RNA from a wide variety of sources - Total RNA can be isolated from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses using this kit.
  • Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array analysis requiring the use of intact RNA.