Human Gene Expression Microarray

human microarray for gene expression OneArray® Human Microarrays are used for gene expression profiling. Researchers can start with RNA from a variety of sources, including: cells; fresh, frozen or FFPE tissues. Read our MAQC technical note to learn about OneArray® microarray performance as compared to other platforms.

Features

  • 29,187 human genome probes and 1088 experimental control probes
  • Highly sensitive 60-mer sense-strand polynucleotide probes
  • Standard 1" X 3" glass slide conforms with many commercial scanners
  • Latest information of human genome content from public domains is used to design the probes that monitor the expression level of corresponding human protein-coding genes
  • Excellent array consistency using our innovative, non-contact microarray spotting technology - PhalanxJet
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Ordering Information
Item Cat. No. Acad. Price Comm. Price Bulk Price
Human Whole Genome OneArray® Microarray v5
HOA_005

Selected Human OneArray Publications
  • Qi HH, Sarkissian M, Hu GQ, Wang Z, Bhattacharjee A, Gordon DB, Gonzales M, Lan F, Ongusaha PP, Huarte M, Yaghi NK, Lim H, Garcia BA, Brizuela L, Zhao K, Roberts TM, Shi Y. Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development. Nature. 2010 Jul 22;466(7305):503-7. (PubMed)
  • Chen H, Sun H, You F, Sun W, Zhou X, Chen L, Yang J, Wang Y, Tang H, Guan Y, Xia W, Gu J, Ishikawa H, Gutman D, Barber G, Qin Z, Jiang Z. Activation of STAT6 by STING is critical for antiviral innate immunity. Cell. 2011 Oct 14;147(2):436-46. (PubMed)
  • Qu Y, Wang J, Ray PS, Guo H, Huang J, Shin-Sim M, Bukoye BA, Liu B, Lee AV, Lin X, Huang P, Martens JW, Giuliano AE, Zhang N, Cheng NH, Cui X. Thioredoxin-like 2 regulates human cancer cell growth and metastasis via redox homeostasis and NF-KB signaling. J Clin Invest. 2011 Jan 4;121(1):212-25. (PubMed)
  • Wu J, Liu Z, Shao C, Gong Y, Hernando E, Lee P, Narita M, Muller W, Liu J, Wei JJ. HMGA2 overexpression-induced ovarian surface epithelial transformation is mediated through regulation of EMT genes. Cancer Res. 2011 Jan 15;71(2):349-59. (PubMed)
  • Fan LC, Chiang WF, Liang CH, Tsai YT, Wong TY, Chen KC, Hong TM, Chen YL. a-Catulin knockdown induces senescence in cancer cells. Oncogene. 2011 Jun 9;30(23):2610-21. (PubMed)
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OneArray® Advantages
  • 100% satisfaction & replacement guarantee
     
  • Outstanding customer and technical support team
     
  • Competes head-to-head with popular platforms
  • Highly competitive pricing  << More>>
     
  • Specific 60-mer oligonucleotide probes
     
  • Uniform, non-contact printing technology

A microarray image - low resolution
B microarray image - high resolution
Figure 1: Post Hybridization of Human OneArray using dual colors, indirect labeling of aRNA. A) shows the full microarray; B) is a magnified region to show clarity and spot morphology.

Probe Set Content
  • Each 60-mer oligonucleotide probe is designed to hybridize to a specific target gene described in the latest public domain sources available from the NCBI Reference Sequence (RefSeq) and Ensembl Databases.

  • The most well-known and studied target genes are the first priority of probe selection.

  • Each microarray contains 1088 performance monitoring control probes for hybridization, sample quality, and labeling reactions. Also included are the ArrayControl™ Sense Oligo Spots by Ambion.

  • Downloadable gene lists, probe sequences, and whole genome GAL files. << More>>
Human OneArray® v5 Probe Set Content
Probe Type Number of Probes
Total Probes 30,275*
Content Probes
  • RefSeq release 38
  • Ensembl release 56
 29,187
Control Probes 1088
*Human OneArray is guaranteed to have > 98% of the total probe content printed.
Table 1 (above): Human Genome Content

Human OneArray® Performance
Table 2 (right) displays results obtained using five Human OneArray v5 DNA Microarrays. 2.5 ug of Brain Reference total RNA (Ambion) was amplified using an Amino Allyl MessageAmp™ Kit (Ambion).

For hybridization, 10 ug of aRNA was labeled with Cyanine 5 fluorescent, and scanned with an Agilent scanner*.
Human OneArray® Whole Genome Hybridization
  Spot Number Spot Percentage Average Signal Intensity
Average Value 24,926 85.4 5,803
CV 2.0% 2.0% 3.8%
Table 2: Human OneArray Repeatability

To learn more about the OneArray advantages, visit The Power of OneArray. << More>>
*Axon scanner settings:
PMT / Power: 600/100;
Filter: F635.Median - B635.Mean >2.6 B635 sd.

Control Features
  Visit our Experimental Controls support page to learn more about how to use the 1,088 experimental control probes on Human OneArray. << More>>