Rat Gene Expression Microarray
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OneArray® Rat Microarrays are used for gene expression profiling. Applications include interrogation into pathways observed in rat disease models and ADMET/DMPK studies. Researchers can start with RNA from a variety of sources, including: cells; fresh, frozen or FFPE tissues.
Features
- 24,358 rat genome probes and 980 experimental control probes
- Comprehensive gene coverage based on RefSeq release 42 and Ensembl release 59
- Superior hybridization performance through isothermal ~60-mer sense-strand probe design
- Excellent data reproducibility with CV < 4%
- High Correlation with Real-Time PCR
- Excellent array consistency using our innovative, non-contact microarray spotting technology - PhalanxJet
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Ordering Information |
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| Item |
Cat. No. |
Acad. Price |
Comm. Price |
Bulk Price |
| Rat Whole Genome OneArray® Microarray |
ROA_001 |
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OneArray® Advantages |
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- 100% satisfaction & replacement guarantee
- Outstanding customer and technical support team
- Competes head-to-head with popular platforms
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- Highly competitive pricing << More>>
- Isothermal ~60-mer probe design
- Uniform, non-contact printing technology
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| A |
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| B |
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| Figure 1: Post Hybridization of Human OneArray using dual colors, indirect labeling of aRNA. A) shows the full microarray; B) is a magnified region to show clarity and spot morphology. |
Probe Set Content |
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- The Rat OneArray probe set is a combination of the RefSeq release 42 and Ensembl release 59 databases.
- Long oligonucleotide probes (~60-mers) were engineered using specific lengths to match melting temperatures for superior hybridization performance.
- Each microarray contains 980 performance monitoring control probes for hybridization, sample quality, and labeling reactions. Also included are the ArrayControl™ Sense Oligo Spots by Ambion.
- Downloadable gene lists, probe sequences, and whole genome GAL files. << More>>
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| Rat OneArray® Probe Set Content |
| Probe Type |
Number of Probes |
| Total Probes |
25,338* |
Content Probes
- RefSeq release 42
- Ensembl release 59
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19,247
5,111 |
| Control Probes |
980 |
*Rat OneArray® is guaranteed to have > 98% of the total probe content printed.
Table 1 (above): Rat Genome Content |
Rat OneArray® Performance |
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Excellent Reproduciblity |
Table 2 (right) displays results obtained using five Rat OneArray DNA Microarrays. 2.5 ug of Rat Brain Reference total RNA (Ambion) was amplified using an Amino Allyl MessageAmp™ Kit (Ambion).
For hybridization, 10 ug of aRNA was labeled with Cyanine 5 fluorescent, and scanned with an Axon scanner*. |
| Rat OneArray® Whole Genome Hybridization |
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Spot Number |
Spot Percentage |
Average Signal Intensity |
| Average Value |
18722 |
76.9% |
2547 |
| CV |
3.25% |
3.25% |
3.9% |
Table 2: Rat OneArray Repeatability
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*Axon scanner settings:
PMT / Power: 600/100;
Filter: F635.Median - B635.Mean >2.6 B635 sd. |
High qPCR Correlation |
To assess OneArray performance, rat microarray data was validated by qPCR on Universal Reference and Brain RNA samples. 40 genes were randomly selected from the linear detection range of the microarray dataset and compared to qPCR results. An excellent correlation to qPCR (R = 0.978) was demonstrated, offering strong assurance of high-fidelity in your microarray studies.
Figure 1, right, shows the log2 ratios of Brain (B) to Universal Reference (A) RNA samples with qPCR and microarray data represented on the x- and y-axes, respectively.
To learn more about the OneArray advantages, visit The Power of OneArray. << More>> |
Figure 2: Rat OneArray qPCR Correlation |
Control Features |
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Phalanx Biotech Group. All rights reserved. © 2012.
