Rice Gene Expression Microarray

rice microarray for gene expression OneArray® Rice Microarrays are used for gene expression profiling. As the world's major food staple, rice has been one of the most studied plant genomes.

Features

  • 21,179 rice genome probes and 824 experimental control probes
  • Comprehensive gene coverage based on RGAP version 6.1 and BGI v2008 databases
  • Superior hybridization performance through isothermal ~60-mer sense-strand probe design
  • Excellent data reproducibility with CV < 6%
  • High Correlation with Real-Time PCR
  • Excellent array consistency using our innovative, non-contact microarray spotting technology - PhalanxJet
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Ordering Information
Item Cat. No. Acad. Price Comm. Price Bulk Price
Rice Whole Genome OneArray® Microarray
RiOA_001

OneArray® Advantages
  • 100% satisfaction & replacement guarantee
     
  • Outstanding customer and technical support team
     
  • Competes head-to-head with popular platforms
  • Highly competitive pricing  << More>>
     
  • Isothermal ~60-mer probe design
     
  • Uniform, non-contact printing technology

A microarray image - low resolution
B microarray image - high resolution
Figure 1: Post Hybridization of Human OneArray using dual colors, indirect labeling of aRNA. A) shows the full microarray; B) is a magnified region to show clarity and spot morphology.

Probe Set Content
  • The Rice OneArray probe set is a combination of the Rice Genome Annotation Project (RGAP) version 6.1 and Beijing Genomics Institute (BGI) version 2008 databases.

  • Long oligonucleotide probes (~60-mers) were engineered using specific lengths to match melting temperatures for superior hybridization performance.

  • Each microarray contains 824 performance monitoring control probes for hybridization, sample quality, and labeling reactions. Also included are the ArrayControl™ Sense Oligo Spots by Ambion.

  • Downloadable gene lists, probe sequences, and whole genome GAL files. << More>>
Rice OneArray® Probe Set Content
Probe Type Number of Probes
Total Probes 22,003*
Content Probes
  • Japonica
  • Indica
 20,302
13,110
Control Probes 824
*Rice OneArray® is guaranteed to have > 98% of the total probe content printed.
Table 1 (above): Rice Genome Content

Rice OneArray® Performance

Excellent Reproduciblity
Table 2 (right) displays results obtained using five Rice OneArray DNA Microarrays. 2.5 ug of Rice Root Reference total RNA was amplified using an Amino Allyl MessageAmp™ Kit (Ambion).

For hybridization, 10 ug of aRNA was labeled with Cyanine 5 fluorescent, and scanned with an Axon scanner*.
Rice OneArray® Whole Genome Hybridization
  Spot Number Spot Percentage Average Signal Intensity
Average Value 18,920 89.3% 3,635
CV 3.20% 3.20% 5.76%
Table 2: Rice OneArray Repeatability
*Axon scanner settings:
PMT / Power: 600/100;
Filter: F635.Median - B635.Mean >2.6 B635 sd.

High qPCR Correlation
To assess OneArray performance, rice microarray data was validated by qPCR on Rice Root Reference and Rice Shoot Reference RNA samples. 66 genes were randomly selected from the linear detection range of the microarray dataset and compared to qPCR results. An excellent correlation to qPCR (R = 0.978) was demonstrated, offering strong assurance of high-fidelity in your microarray studies.


Figure 1, right, shows the log2 ratios of Brain (B) to Universal Reference (A) RNA samples with qPCR and microarray data represented on the x- and y-axes, respectively.


To learn more about the OneArray advantages, visit The Power of OneArray. << More>>		 rice_qPCR
Figure 2: Rice OneArray qPCR Correlation

Control Features
  Visit our Experimental Controls support page to learn more about how to use the 824 experimental control probes on Rice OneArray. << More>>