Yeast Gene Expression Microarray

yeast microarray for gene expression OneArray® Yeast Microarrays are used for gene expression profiling. Yeast serves as a model organism for genetic manipulation and expression studies have provided a wealth of knowledge to researchers.

Features

  • 6,958 yeast genome probes, and 684 experimental control probes
  • Highly sensitive 70-mer sense-strand polynucleotide probes
  • 1" X 3" (25mm x 75mm) standard-format glass slide
  • Probe set is base on Operon Yeast Genome Array-Ready Oligo Set (AROS) v1.1 and Yeast Brown Lab Oligo Extension (YBOX) v1.0
  • Each probe is spotted onto the array in a highly consistent manner using an innovative non-contact spotting technology adapted and perfected by Phalanx Biotech for microarray manufacturing.

Ordering Information

Item Cat. No. Acad. Price Comm. Price Bulk Price
Yeast Whole Genome OneArray® Microarray YOA_001

OneArray® Advantages

  • 100% satisfaction & replacement guarantee
     
  • Outstanding customer and technical support team
     
  • Competes head-to-head with popular platforms
  • Highly competitive pricing  << More>>
     
  • Specific 70-mer oligonucleotide probes
     
  • Uniform, non-contact printing technology

A yeast microarray image - low resolution
B yeast microarray image - high resolution
Figure 1: Post Hybridization of Yeast OneArray using dual colors, indirect labeling of aRNA. A) shows the full microarray; B) is a magnified region to show clarity and spot morphology.

Probe Set Content

  • Each 70-mer probe is designed within 750 bases from the 3'end of the open reading frame
    and is selected from the Operon Yeast Genome Array-Ready Oligo Set (AROS)* v1.1 or Yeast
    Brown Lab Oligo Extension (YBOX) v1.0.
     
  • 684 performance monitoring control probes for hybridization, sample quality, and labeling reactions.

  • Also included are the ArrayControl™ Sense Oligo Spots by Ambion.

  • Downloadable gene lists, probe sequences, and whole genome GAL files. << More>>

*: AROS contains 6388 probes designed from the set of open reading frames obtained from the Saccharomyces Genome Database (June 2004). An additional 570 probes were selected from YBOX which was designed by P. Brown lab at Stanford based on Saccharomyces Genome Database (July 2005). 

Yeast OneArray® Probe Content
Probe Type Number of Probes
Yeast Genome 6,958 (total)**
Saccharomyces cerevisiae open reading frames 6,787
Saccharomyces cerevisiae non-coding (RNAs including tRNAs, snoRNAs, rRNAs, etc) 171
Experiment control 684 (total)
Alignment controls 202
Positive & Negative controls 338
Extrinsic controls 144
**Yeast OneArray is guaranteed to have >98% of the total probe content printed.
Table 1 (above): Human Genome Content

Yeast OneArray® Performance

Array to array consistency is essential for accurate comparison of various experimental samples. In this production of Yeast OneArrays, typical total median CV of <10% and average R^2 of ≥0.97 were obtained.



To learn more about the advantages of OneArray, visit The Power of OneArray.
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Figure 2 (right): Array-to-array log ratio correlation for Yeast OneArray®

Control Features

  Visit our Experimental Controls support page to learn more about how to use the 684 experimental control probes on the Yeast OneArray. << More>>