Microarray Hybridization Chambers

OneArray® Hyb Chamber Advantages


  • Custom-fit to OneArray Whole Genome and miRNA microarrays.  
  • Leak-free seal allows rotational mixing to ensure uniform hybridization.  
  • No drying of arrays at elevated temperatures and long equilibration times.
  • Compatible with most microarrays printed on standard 1"x3" glass slides.  

OneArray® Hybridization Chambers

Full Length Chamber for Whole Genome OneArray®
Product Description
Our Full Length Chamber is designed to fit the Whole Genome OneArray product line. It features a self-adhesive backing and two sealable loading ports.

Download: OneArray® Full Length Chamber Guide
View: Hybridization Chamber Tutorial Videos video
 
Product Specifications
OneArray® Full Length Chamber
RNA sample per array ~ 4-5 ug
Volume 180 +/- 10 uL
Cost $99 / 10 chambers

Double Chamber for microRNA OneArray®
Product Description
Our Double Chamber is designed to fit the microRNA OneArray product line. Each of the two chambers feature a self-adhesive backing and two sealable loading ports.

Download: OneArray® Double Chamber Guide
View: Hybridization Chamber Tutorial Videos video
 
Product Specifications
OneArray® Double Chamber
RNA sample per array ~ 1-2 ug
Volume 70 +/- 10 uL
Cost $99 / 10 chambers

Performance

Experiment to experiment consistency is critical for generating reliable data, and the hybridization chambers provide an easy and systematic method to produce consistent results.

To demonstrate the performance of the hybridization chambers, two samples were compared on each of the Human and Mouse OneArray® microarrays using the hybridization chambers. In total, 12 microarrays and hybridization chambers were used. The pairwise correlation and scatterplot regression values indicate the OneArray® FL Hybridization Chambers provide excellent repeatability.
Tables 1 & 2: Pairwise correlation of raw intensities from samples performed on the A) Human OneArray®, and B) Mouse OneArray®, in technical triplicates using FL Hybridization Chambers. TRT, CTR denotes 'treated' and 'control' samples, respectively. Number suffix refers to the technical repeat. Figures 1 & 2: Log2 signal reproducibility comparing a representative pair of technical repeats performed using FL Hybridization Chambers for samples on the A) Human OneArray, and B) Mouse OneArray. Axes represent log2 transformed raw intensities. Figures are produced from the same data as Tables 1 & 2.

A) Human OneArray® with FL Hybridization Chambers
 
TRT.1
TRT.2
TRT.3
CTR.1
CTR.2
CTR.3
TRT.1
1.000
0.989
0.978
     
TRT.2
 
1.000
0.993
     
TRT.3
   
1.000
     
CTR.1
     
1.000
0.992
0.988
CTR.2
       
1.000
0.989
CTR.3
         
1.000
hoa-scatter

B) Mouse OneArray® with FL Hybridization Chambers
 
TRT.1
TRT.2
TRT.3
CTR.1
CTR.2
CTR.3
TRT.1
1.000
0.989
0.996
     
TRT.2
 
1.000
0.988
     
TRT.3
   
1.000
     
CTR.1
     
1.000
0.989
0.986
CTR.2
       
1.000
0.994
CTR.3
         
1.000
moa-scatter