Kreatech ULS™ microRNA Labeling Kit

Features
  • Labeling reaction is scalable.
  • Labels all microRNA species, independent of modifications (e.g. piRNAs and plant microRNAs that are 2' O-methylated at the 3' end).
  • Label your microRNA samples in only 15 minutes.
  • Labeling is a non-enzymatic reaction, producing consistent results.
  • Optimized kits and protocols ensure highly sensitive and reproducible results.
Quick Links
The Kreatech ULS™ microRNA product is approved for use with the OneArray® microRNA platform.

Ordering Information
Item Cat. No. Kit Size Price (USD)*
ULS™ microRNA Labeling Kit (with Cy3 & Cy5) EA-036 10 dual labelings $ 895
ULS™ microRNA Labeling Kit (with Cy3) EA-037 20 single labelings $ 895
ULS™ microRNA Labeling Kit (with Cy5) EA-038 20 single labelings $ 895
  *microRNA labeling kits must be purchased with microRNA OneArray microarrays. << More>>

Technology Overview
Universal Linkage System (ULS™): A versatile, chemical labeling technology.

The proprietary ULS™ technology provides the basis of Kreatech's broad range of labeling-based applications, including microRNA microarrays. ULS labeling is based on the stable binding properties of platinum(II) to nucleic acids and proteins. The ULS molecule consists of a monofunctional platinum complex coupled to a detectable molecule of choice. The platinum atom forms a coordinative bond, firmly coupling the detectable molecule to the biomolecule of choice: DNA, RNA or protein. ULS labels DNA and RNA by binding to N-7 of guanine. In proteins, ULS binds to sulfur-containing side chains of methionine, and cysteine, and to a nitrogen atom in histidine.

Key Figures
ULS without enzyme
Figure 1: Schematic Overview of the ULS Technology.

 
DNA/RNA Target
Figure 2: DNA/RNA Target Labeling Location.

 
A B
Figure 3: Small molecules are labeled as efficiently as large molecules. ULS labeling is independent of nucleic acid fragment length. (A) Molecules of different fragment lengths were labeled, and the Degree of Labeling was determined. (B) Total RNA was labeled, and separated in 2 fractions, i.e. small RNA, and total RNA without small RNA. Shown are the Degree of Labeling values of the individual fractions.

Benefits
  • Stable in aqueous solutions (enabling automation) and stored at 4°C
  • Uniform labeling of native DNA, RNA and oligonucleotides as well as enzymatically amplified nucleic acids (e.g. PCR, WGA, aRNA products)
  • Size independent labeling of nucleic acids; e.g. ranging from microRNA (20mer) to genomic DNA (>20kb)
  • Suitable for the labeling of 3' / 5' modified nucleic acids (e.g. plant and germ-line microRNA)
  • Suitable for the labeling of native bacterial mRNA
  • Efficiently labels nucleic acids from archival material such as DNA/RNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue
  • Optimal removal of unreacted label using KREApure™ columns