mRNA qPCR Services

Gene Expression qPCR Service Highlights

  • We offer TaqMan or SYBR Green chemistries according to your needs.  
  • Our services include complete primer design and optimization.  
  • We will also identify suitable reference genes.  
  • And we will deliver completely analyzed data!  

OneArray® qPCR Service Features

  • OneArray qPCR gene expression profiling is available for any species with sequence data. We have extensive experience with both TaqMan and SYBR Green qPCR assay design.

  • Our qPCR service workflow includes RNA QC, primer design, primer optimization, reference gene testing, and data analysis.

  • Our primer design and optimization workflow includes testing for the correct product size and specificity via gel electrophoresis and melting curve analysis. We will also test the amplification efficiency of each primer pair using standard curves across a dilution series.

  • Traditional reference genes such as GAPDH and beta-Actin are not suitable in all experiments. We will identify reference genes in your experiment by measuring the expression of a group of candidate reference genes and choosing the most stable gene(s).

  • If you would like to learn more about our qPCR services, please call us at 877-730-3887, or click HERE to submit an inquiry through our website.

Recent OneArray® Publications

  • In silico and experimental analyses predict the therapeutic value of an EZH2 inhibitor GSK343 against hepatocellular carcinoma through the induction of metallothionein genes Oncoscience. doi:10.18632/oncoscience.285. (Link)
  • Optimizing a Male Reproductive Aging Mouse Model by d-Galactose Injection International Journal Of Molecular Sciences. doi:10.3390/ijms17010098. (Link)
  • A novel cell-penetrating peptide suppresses breast tumorigenesis by inhibiting ?-catenin/LEF-1 signaling Scientific Reports . doi: 10.1038/srep19156. (Link)
  • Gene expression profiling identifies candidate biomarkers for active and latent tuberculosis Bmc Bioinformatics. doi: 10.1186/s12859-015-0848-x. (Link)
  • Upregulation of MicroRNA-19b predicts good prognosis in patients with hepatocellular carcinoma presenting with vascular invasion or multifocal disease Bmc Cancer. DOI 10.1186/s12885-015-1671-5. (Link)
See all publications