Microarray Sensitivity and Dynamic Range

OneArray® Shows Exceptional Sensitivity and Dynamic Range

Spike-in Control Probes for Human OneArray

Phalanx Biotech Human OneArray exhibits excellent sensitivity – as low as 0.050 pM – without sacrificing dynamic range. This allows you to obtain meaningful differential gene expression data for thousands of relevant genes.

Sensitivity and dynamic ranges define the lowest and highest boundaries of signal intensity obtained from the microarray that can accurately reflect the differences in amounts of targets present in a sample.

Many factors such as probe design, composition of hybridization buffer, surface chemistry, etc., can influence the sensitivity and dynamic range of the microarray.

Since the molecular amount of any target within an in-vivo derived sample cannot be determined quantitiatively in a straightforward approach, external spike-in targets are generally used in these experiements.

Human OneArray has incorporated the following two commercially available spike-in systems that are commonly used on self-printed microarrays:

  • SpotReport® Oligo Array and Alien® Oligo Array
    Validation Systems by Stratagene
  • Array Control Sense Oligo Spots by Ambion

Additionally, we have designed and incorporated into our Human OneArray product, an internally developed spike-in system with similar functions.

Customers can also purchase the associated RNA Spike as positive controls allowing the monitoring of quality of target preparation and determination of the sensitivity of hybridization. For detail product information and instruction manual, please contact Stratagene and Ambion directly.

Fig 1 (to right) - A, B & C: Sensitivity of Control Probes
Results from all three spike-in systems show that the lowest signal intensity and the dynamic range that can be reliable detected are approximately 0.05 pM and 2-3 orders of magnitude, respectively.