Next Generation Sequencing (NGS) is a powerful tool to observe the genetic map of your organism of choice. Look for SNP variation or causative loci that underlie your favorite phenotype. Determine how drug manipulation affects expression of coding regions across the genome. Discover novel transcripts and even explore the genetic makeup of an organism previously unmapped.
NGS allows researchers to acquire a range of information in genome research, transcriptomics, epigenetics, and metagenomics, among others.
Phalanx Biotech Group provides NGS services using Illumina’s HiSeq 2500 and MiSeq platforms. Our staff will help you with experimental design, library prep and sequencing questions along the way.
Our NGS service offerings include:
RNA-Seq Service
RNA sequencing is a type of next-generation sequencing used to target regions of the genome that are transcribed into messenger RNA (mRNA) and may subsequently translate into protein. RNA-seq is used to identify and quantify transcriptional regulation of the genome.
Protocols specific to the library preparation stage are used to isolate mRNA and rid samples of rRNA, which comprises approximately 80% of the RNA pool.
Typical sequencing configurations:
| Configuration | mRNA-seq |
| Library Preparation Protocol | Strand-specific, polyA Selection, paired-end |
| Recommended Throughput | 20-40 million reads per sample |
| Platform | Illumina HiSeq |
*Sequencing configuration is project specific.
Sample submission requirements for RNA-Sequencing:
| SAMPLE TYPE | AMOUNT | RIN | CONCENTRATION |
|---|---|---|---|
| Total RNA in Rnase-free water, on dry ice | 2 ug (min > 500 ng) | 6.0 | 50 ng/uL |
RNA-Seq Applications:
RNA-seq may be used for gene expression profiling, identification of novel transcripts, identification of alternative splice events and transcriptome assembly.
Gene expression profiling by way of RNA-seq is utilized in drug development experiments, cancer research, gut microbiome studies and many more. Comparative analysis of transcript abundance between experimental groups provides unique insight into the interplay between gene and environment.
Contact us for an estimate.
Small RNA-Seq Service
Small RNA sequencing is used to target small RNA molecules including: miRNA, ncRNA, snRNA, etc.
Common sequencing configurations:
| Configuration | small RNA-seq |
| Library Preparation Protocol | Customizable size selection using small RNA library prep kits |
| Recommended Throughput | 7-10 million reads per sample |
| Platform | Illumina NextSeq |
Sample submission requirements for Small RNA-Sequencing:
| Sample Type | Minimum Amount | RIN | Minimum Concentration |
|---|---|---|---|
| Total RNA | 200 ng | >7.0 | 20 ng/uL |
Small RNA-Seq Applications
Sample submission requirements:
| Sample Type | Minimum Amount | Minimum Concentration |
|---|---|---|
| RNA | 1 ug | 36 ng/uL |
- RNA QC Requirements
- A260/280 > 1.8
- A260/230 = 2.0~2.2
- RIN > 7
- Recommended Sequencing Specifications
- Paired end 2x100 bp
- 10M, 20M, 40M, 75M, or 100M reads
ChIP-Seq Sample submission requirements:
| Sample Type | Minimum Amount | Minimum Concentration |
|---|---|---|
| DNA | 5 ng | 1 ng/uL |
RNA QC Requirements
- A260/280 > 2
- A260/230 = 2.0~2.2
Recommended Sequencing Specifications
- Single end 1x50 bp
- 20M, 40M, 75M, or 100M reads
Sample submission requirements:
| Sample Type | Minimum Amount | Minimum Concentration |
|---|---|---|
| DNA | 12.5 ng | 1.5 ng/uL |
RNA QC Requirements
- A260/280 > 2
- A260/230 = 2.0~2.2
Recommended Sequencing Specifications
- Paired end 2x300 bp
- 100K coverage
Sample submission requirements:
| Sample Type | Minimum Amount | Minimum Concentration |
|---|---|---|
| DNA | 12.5 ng | 1.5 ng/uL |
RNA QC Requirements
- A260/280 > 2
- A260/230 = 2.0~2.2
Recommended Sequencing Specifications
- Paired end 2x300 bp
- 100K coverage
Brochure:
For in depth information about each sequencing type and data analysis package, please download our brochure.
Highlights:
- Complete Workflow: We are able to perform sample extraction, sequencing, and customized data analysis for a complete NGS service package.
- Low Yield Samples: If your sample type has limited RNA/DNA, then please contact us to learn about our specialized workflows for low yield samples.
- Wide Range of Species: We’ve processed samples from species with and without reference genomes (human, mouse, rat, hamster, bacteria, etc…)
Downloadable Resources:
Blog Post:
Read our latest blog post with tips for choosing a good NGS service provider.