We are a microarray service lab that brings you the very best in Gene Expression
Profiling Services using our proprietary OneArray® Platform, which is continuously
validated, tested and updated. Since we design and manufacture our own microarrays
in-house, we are able to offer affordable pricing on our microarray service.
Just send us your RNA or your tissue/cell/serum samples, and we will do the rest! We
perform RNA extraction, QC, microarray service and data analysis..
Highlights
- OneArray® miRNA expression profiling is available for the following species: Human (HmiOA v7), Mouse & Rat (MRmiOA v7), and Model Plant species (MPmiOA v3)
- Probes contain miRNA-specific sequences and a proprietary spacer designed to enhance the hybridization sensitivity to miRNAs.
- Microarray service provider for 10+ years.
- Outstanding technical support from microarray experts.
- Professional lab technicians with 70+ years of combined experience.
- Our complete microarray service workflow includes RNA QC, miRNA enrichment, labeling, hybridization, scanning, data extraction, and data analysis.
Overview
- Species available:
| Species | OneArray Platform | No. of miRNA Probes |
|---|---|---|
| Human | HmiOA v7 | 2,548 |
| Mouse & Rat | MRmiOA v7 | 1,481 Mouse 760 Rat |
| Model Plants | MPmiOA v3 | 1,018 from 7 species |
- Workflow:
| Step | Method | QC metrics |
|---|---|---|
| RNA isolation (optional) | Trizol/RNeasy/etc. | Need > 3ug |
| RNA QC | NanoDrop | 260/230 > 1, 260/280 > 1.6 |
| Agilent Bioanalyzer | RIN > 5 | |
| miRNA enrichment | Nanosep® 100k Columns | See below |
| miRNA labeling | ULS™ Small RNA Labeling Kit | Internally validated thresholds for labeling efficiency measured with NanoDrop |
| Hybridization | Phalanx Internal Protocol | See below |
| Data extraction | Agilent Extended Dynamic Range | Internally validated metrics for hybridization efficiency using control microarray probes |
- Data Analysis:
- Data normalization
- Identify differentially expressed miRNAs (unlimited statistical comparisons)
- Principal components analysis
- Hierarchical clustering
- Gene target prediction of differentially expressed miRNAs
- Gene Ontology and Pathway enrichment analyses of predicted gene targets
Downloadable Resources
BROCHURES
SAMPLE REPORTS
SAMPLE SUBMISSION
TECH NOTES