If you are interested in gene or miRNA expression analysis you’ve probably weighed the pros and cons of different genomic platforms on which to carry out your experiment. While qPCR is a method that is often used to validate expression data from array experiments, it is also used as a direct method for measuring gene expression or transcript abundance. qPCR is particularly a great choice if you are interested in a specific subset of genes or miRNAs and/or have a specific functional pathway in mind. Here at Phalanx Biotech, we have a long history of providing reliable and quality qPCR service to our customers. Our qPCR features include primer design, choice of TaqMan or SYBR Green chemistries, data analysis and assistance from a qPCR expert.
It is often the case that researchers are seeking answers to complex questions that are compounded by many variables. Under some circumstances, standard experimental protocols are not sufficient to answer these questions. Our scientists are skilled at assay optimization and provide customized qPCR workflows to meet the customer’s experimental goals.
Below, is a case study of a qPCR project carried out by our scientists where customized workflows were designed to meet the specific research goals of the client.
Case Study – qPCR Service for miRNA Analysis
Client Situation: A research and development company wished to analyze miRNA expression for multiple targets from >50 patient serum samples. Serum is characterized by relatively low levels of RNA, leading to increased difficulty in identifying a large spectrum of the mature miRNAs in a sample.
Phalanx-driven research solution: Through the use of cutting-edge technology and novel insight, our qPCR PhD-level scientist developed a workflow to meet the customer’s needs. This workflow included an exogenous spike-in for normalization and employed the use of TaqMan Advanced miRNA qPCR protocol in order to isolate and quantify the expression of mature miRNAs from serum samples.
Our team of scientists analyzed the data and delivered a custom qPCR Analysis Report that contained the normalized data, data comparison to a reference sample and a heat map for visualization of differential expression of targets between samples.
Results: Through the use of custom-tailored methodologies we were able to detect miRNA expression from low-level RNA samples. We provided the client with genomic data which helped them to meet their experimental goals. The client was able to move forward with their project and plan to contact us for their next genomic study.
Please check out our qPCR Services.